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Characterization of the complementarity determining regions in immunoglobulin by the 6-mers which are most variable in the HIV-1 genome.




 

Int Conf AIDS. 1994 Aug 7-12;10(2):84 (abstract no. PA0218). Unique

OBJECTIVE: To estimate the characteristics of complementarity determining regions (CDRs) in immunoglobulin (Ig) gene which are essential to interact with variable epitopes of HIV-1 proteins. METHODS: Using the data base DDBJ we analyzed CDRs in human and rodent Ig genes by the purine (u)/pyrimidine (y) 6-mers [uuyuuy], which are most variable in the HIV-1 genome (Doi, H., Proc. Natl. Acad. Sci. USA, 1991, 88, 9282-9286), and their complementary 6-mers [uyyuyy]. RESULTS: TABULAR DATA, SEE ABSTRACT VOLUME. The 6-mers [uuyuuy] and/or [uyyuyy] characteristically and differently appeared between in human and rodent Ig genes or in each CDRs of H-chain and L-chain. They most frequently appeared (87.7%) in CDR3 of human H-chain, but infrequently in CDR2 of human H-chain (29.6%). In contrast, they frequently appeared in CDR2 of rodent L-chain (81.3%), and infrequently in CDR1 of rodent H-chain (30.9%). Codon frames in the 6-mers also differed between the two species. DISCUSSION AND CONCLUSIONS: The characteristics of CDRs are different between in human and in rodents Ig. The results suggest that chimeric anti-HIV antibodies having CDRs from rodents would not effectively operate or would cause any side effect in human because of idiotype network.

Animal Base Sequence Epitopes/IMMUNOLOGY *Genes, Immunoglobulin Genome, Viral Human HIV Antibodies/*BIOSYNTHESIS/GENETICS HIV-1/*GENETICS/*IMMUNOLOGY Immunoglobulins, Heavy-Chain/BIOSYNTHESIS/GENETICS Immunoglobulins, Light-Chain/BIOSYNTHESIS/GENETICS Rodentia *Variation (Genetics) ABSTRACT



 




Information in this article was accurate in December 30, 1994. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.