Int Conf AIDS. 1994 Aug 7-12;10(2):115 (abstract no. PA0340). Unique
OBJECTIVE: Todoxin is a novel antiviral and immunostimulating
preparation based upon an ion carrier molecule with specific binding
affinities and a carrier specific substrate formulation. Todoxin's
formulation is unique in three aspects: 1. It comprises a very broad
spectrum of physiologically active, cis-oriented and water soluable
carbohydrates, carboxylic acids, vitamins, fat soluable vitamins,
coenzymes and enzymes such as Amylases, Phosphatases and Catalases. 2.
It contains both agonic and antagonic agents such as Ca and Fe in
physiologically relevant concentrations. 3. It contains a spectrum of
minerals in permanent solution. Induction of desired cell physiologic
effects in vitro such as increase in cellular exchange diffusion, uptake
of substrates, metabolites, oxydative phosphorylation, mitogen response
of RET cells, have previously been described by author. We report here:
(a) Todoxin's efficacy to increase Natural Killer (NK) cell activity in
vitro. (b) Todoxin's efficacy to inhibit HIV transfection in vitro.
METHODS: (a) Peripheral blood lymphocytes were treated with Todoxin for
17 h and tested for killing of B cell lymphoma cell line DAUDI in a 3 h
Cr51-release assay. Treatments were done in medium or IL-2 (10 U/ml).
The highest Todoxin concentration was a final dilution of 1:10 with 4
consecutive dilution steps 1:10. (b) HIV infected CEM-IW cells were
incubated 7 days with fraction of Todoxin. Concurrent studies were
performed on different sample concentrations. The lowest sample
concentration was 6.0 x 10-1 ug/ml with increasing steps of Log10.
RESULTS: (a) Todoxin increases NK killing to the same levels as IL-2
does. (b) Todoxin prevents HIV infection in vitro 100% as per uninfected
untreated control culture. CONCLUSION: The described results may in part
explain the potent therapial effects of Todoxin seen in viral disease
including slow virus infection, retrovirus and virus associated
carcinogenesis. TABULAR DATA, SEE ABSTRACT VOLUME.
Adjuvants, Immunologic/ADMINISTRATION & DOSAGE/CHEMISTRY/ *PHARMACOLOGY
Antiviral Agents/ADMINISTRATION & DOSAGE/CHEMISTRY/*PHARMACOLOGY
Cytotoxicity, Immunologic/DRUG EFFECTS Human HIV/*DRUG
EFFECTS/GENETICS HIV Infections/PREVENTION & CONTROL In Vitro
Interleukin-2/PHARMACOLOGY Killer Cells, Natural/DRUG
EFFECTS/IMMUNOLOGY Transfection/DRUG EFFECTS Tumor Cells,