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Additional anti-HIV activity of antisense with interferons.


Int Conf AIDS. 1994 Aug 7-12;10(2):113 (abstract no. PA0331). Unique

OBJECTIVES: Antisense phosphorothioate oligodeoxynucleotide against rev (S-anti-rev) has been shown to inhibit HIV expression. Interferons (INFs) (alpha, beta, gamma) are also known to regulate HIV expression through many mecha-nisms including interaction with RRE (rev responsive element). METHODS: Chronically HIV-infected T-cell line, H9 cells (H9/HIV), and promonocyte cell line, U1 cells, were cultured for 5 days in 96-well culture plate with or without the compounds. Culture supernatants were collected and tested for p24 gag by ELISA. RESULTS: In H9/HIV, S-anti-rev and INFs exhibited significant anti-HIV activity and the combination of S-anti-rev and INFs resulted in additional anti-HIV activity without addition of their toxicity. In U1 cells, interferon gamma augmented the viral production, which could be inhibited by S-anti-rev. DISCUSSION AND CONCLUSION: The combination of S-anti-rev and INFs could be a potent remedy for AIDS and related disorders.

Acquired Immunodeficiency Syndrome/THERAPY Antiviral Agents/*TOXICITY/THERAPEUTIC USE Cell Line Drug Synergism Gene Products, gag/ANALYSIS/BIOSYNTHESIS *Genes, rev Human HIV/*DRUG EFFECTS/GENETICS/METABOLISM HIV Core Protein p24/ANALYSIS/BIOSYNTHESIS Interferons/*TOXICITY/THERAPEUTIC USE Oligonucleotides, Antisense/*TOXICITY/THERAPEUTIC USE T-Lymphocytes Thiophosphoric Acid Esters ABSTRACT


Information in this article was accurate in December 30, 1994. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.