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NLM AIDSLINE

The highly conserved aspartic acid residue between hypervariable regions 1 and 2 of human immunodeficiency virus type 1 gp120 is important for early stages of virus replication.




 

J Virol. 1995 Jan;69(1):538-42. Unique Identifier : AIDSLINE

Between hypervariable regions V1 and V2 of human immunodeficiency virus type 1 (HIV-1) gp120 lies a cluster of relatively conserved residues. The contribution of nine charged residues in this region to virus infectivity was evaluated by single-amino-acid substitutions in an infectious provirus clone. Three of the HIV-1 mutants studied had slower growth kinetics than the wild-type virus. The delay was most pronounced in a mutant with an alanine substituted for an aspartic acid residue at position 180. This aspartic acid is conserved by all HIV-1 isolates with known nucleotide sequences. Substitutions with three other residues at this position, including a negatively charged glutamic acid, all affected virus infectivity. The defect identified in these mutants suggests that this aspartic acid residue is involved in the early stages of HIV-1 replication.

Amino Acid Sequence Animal Aspartic Acid/GENETICS/*PHYSIOLOGY Cells, Cultured Conserved Sequence HIV Envelope Protein gp120/GENETICS/*PHYSIOLOGY HIV-1/GENETICS/*PHYSIOLOGY Molecular Sequence Data Mutagenesis, Site-Directed Support, U.S. Gov't, Non-P.H.S. Support, U.S. Gov't, P.H.S. *Virus Replication/GENETICS JOURNAL ARTICLE



 




Information in this article was accurate in March 30, 1995. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.