Invest Ophthalmol Vis Sci. 1995 Jan;36(1):254-8. Unique Identifier :
PURPOSE. To understand better the immunopathology of HTLV-I uveitis by
investigating the clonality of HTLV-I-infected T-cell clones. METHODS.
Eleven T-cell clones were established from the aqueous humor (six
clones) and the peripheral blood (five clones) of a patient with HTLV-I
uveitis, and the clonality of the HTLV-I-infected T cells was
investigated by sequencing the T-cell receptor (TCR) alpha gene after
the amplification of TCR alpha cDNA using an adaptor-ligation method and
reverse transcriptase-polymerase chain reaction (RT-PCR). RESULTS. TCR
alpha use was different for each of 11 T-cell clones, encompassing eight
different HTLV-I-infected T-cell clones (four from the aqueous humor and
four from peripheral blood) and three HTLV-I-negative T-cell clones.
CONCLUSIONS. This study demonstrated polyclonal use of TCR alpha for
HTLV-I-infected T cells in the ocular lesion and the peripheral blood.
Results suggested that these T cells are not precursors of the leukemic
cells associated with malignant transformation. Instead, they might be
randomly infected with HTLV-I in the process of HTLV-I uveitis.
Amino Acid Sequence Base Sequence Clone Cells DNA/ANALYSIS Human
HTLV-I/*PHYSIOLOGY HTLV-I Infections/IMMUNOLOGY Molecular Sequence
Data Polymerase Chain Reaction Receptors, Antigen, T-Cell,
alpha-beta/GENETICS/*IMMUNOLOGY Support, Non-U.S. Gov't
T-Lymphocytes/*VIROLOGY Uveitis/IMMUNOLOGY/VIROLOGY JOURNAL ARTICLE