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The role of calcium and N-linked glycans in the oligomerization and carbohydrate binding properties of human immunodeficiency virus external envelope glycoprotein [published erratum appears in Glycoconj J 1994 Oct;11(5):500]




 

Glycoconj J. 1994 Apr;11(2):73-9. Unique Identifier : AIDSLINE

Envelope glycoproteins of human immunodeficiency virus (gp120 and gp41) occur as oligomers. Here, we show by gel filtration analysis that gp120 oligomerization in vitro is calcium- and temperature-dependent. Recombinant gp120 (rgp120) species were recovered as monomers at 20 degrees C in the absence of calcium, but as tetramers at 37 degrees C in 10 mM CaCl2. Under the latter condition, N-glycanase-deglycosylated rgp120 formed hexamers. Relative to intact rgp120, which has been reported to display carbohydrate-binding properties for N-acetyl-beta-D-glucosaminyl and mannosyl residues, deglycosylation enhanced rgp120 specific binding to mannose-divinylsulfone-agarose, para-aminophenyl-beta-D-GlcNAc-agarose and fetuin-agarose matrices. Taken together, these results rule out the role of homologous lectin-carbohydrate interactions via N-linked glycans in the rgp120 oligomerization, even though its lectin properties may also be calcium-dependent. Deglycosylation may unmask domains of rgp120 polypeptide backbone that independently play a role either in rgp120 lectin activity or in calcium-dependent oligomerization.

Calcium/PHARMACOLOGY Chromatography, Gel Comparative Study HIV/*METABOLISM HIV Envelope Protein gp120/*CHEMISTRY/METABOLISM HIV Envelope Protein gp41/*CHEMISTRY/METABOLISM Kinetics Lectins Macromolecular Systems Polysaccharides/*METABOLISM/PHARMACOLOGY Recombinant Proteins/CHEMISTRY/METABOLISM Support, Non-U.S. Gov't Thermodynamics JOURNAL ARTICLE



 




Information in this article was accurate in April 30, 1995. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.