Biotherapy. 1993-94;7(2):125-36. Unique Identifier : AIDSLINE
Supernatants derived from CD8+ lymphocytes treated with mycobacterial
components, or the partially purified carbohydrates from these
supernatants, increased the production of IL-4 and IL-6 by mononuclear
cells. The addition of anti-IL4 or anti-IL6 antibodies to LPS stimulated
MN cells incubated with supernatants from CD8+ lymphocytes or
carbohydrates resulted in the restoration of other cytokine production
by these MN cells. Recombinant IL-4 and IL-6 on their own suppressed the
production of IL-1 beta, TNF-alpha, IL-2 and IFN-gamma by mononuclear
cells. Such suppression could be reversed with antibodies to IL-4 and
IL-6. The addition of rIL-4 and rIL-6 did not increase the suppression
of cytokine production induced by suppressor supernatants or
carbohydrates. Interleukin 4 decreased the production of IL-6 by MN
cells; whilst IL-6 suppressed IL-4 production in a dose dependent
manner. Both effects could be reversed with the appropriate antisera.
Our results suggest that mycobacteria could evade host immunity by
inducing the production of IL-4 and IL-6 by host mononuclear cells.
These cytokines, in turn, would suppress the production of other
cytokines necessary for effective cellular immunity.
Carbohydrates/PHYSIOLOGY Cytokines/*BIOSYNTHESIS CD8-Positive
Lipopolysaccharides/PHARMACOLOGY Mycobacterium/*IMMUNOLOGY Recombinant
Proteins/PHARMACOLOGY Support, Non-U.S. Gov't Suppressor Factors,
Immunologic/*PHYSIOLOGY JOURNAL ARTICLE