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Multisite evaluation of four anti-HIV-1/HIV-2 enzyme immunoassays. Australian HIV Test Evaluation Group.


J Acquir Immune Defic Syndr Hum Retrovirol. 1995 Apr 1;8(4):411-9.

The performance of four enzyme immunoassays, manufactured by Abbott, Diagnostics Pasteur, Genetic Systems, and Organon Teknika, for the combined detection of anti-human immunodeficiency virus type 1 (HIV-1) and anti-HIV-2, was examined in a multisite evaluation. The collaborative efforts of 7 Australian Red Cross Blood Transfusion and 12 Australian Public Health Laboratories minimized potential biases in data by providing large numbers of anti-HIV-1-negative and -positive samples. Sensitivity was estimated using samples that were positive for anti-HIV-1 from individuals known to be infected and seroconversion samples. Sensitivity estimates in the four assays were 99.71, 99.94, 99.49, and 99.68%, respectively. Specificity was measured using fresh, sequential blood donations and samples with previous false-positive reactions in other assays. Specificity estimates from blood donations were 99.92, 99.46, 99.67, and 99.85%, respectively. The data were analyzed further using the delta statistic, which distinguishes the performance of assays of similar sensitivity and specificity by providing a measure of how well results in a population of positive or negative samples are removed from the assay's cutoff value.

Australia Comparative Study False Positive Reactions Human HIV Antibodies/*ANALYSIS HIV Infections/*DIAGNOSIS HIV Seronegativity/IMMUNOLOGY HIV Seropositivity/IMMUNOLOGY HIV-1/*IMMUNOLOGY HIV-2/*IMMUNOLOGY *Immunoenzyme Techniques Predictive Value of Tests Reagent Kits, Diagnostic Sensitivity and Specificity Support, Non-U.S. Gov't CLINICAL TRIAL JOURNAL ARTICLE MULTICENTER STUDY


Information in this article was accurate in June 30, 1995. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.