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Effect of hyperbaric oxygen on tissue distribution of mononuclear cell subsets in the rat.




 

J Appl Physiol. 1994 Nov;77(5):2355-9. Unique Identifier : AIDSLINE

In a previous study we found a significant temporary decrease in the ratio of CD4/CD8 (helper, inducer/suppressor, cytotoxic) T lymphocytes in the peripheral blood of healthy human volunteers after exposure to a single commonly used profile of hyperbaric oxygen (HBO). The transient nature of the changes suggested redistribution of T-cell subsets. The purpose of the present study was to verify such a redistribution and to locate possible target organs in an animal model. A single exposure of rats to HBO (0.28 MPa) induced a highly significant rapid decrease in the CD4/CD8 ratio in peripheral blood count (P < 0.0001), confirming our previous findings in humans. HBO also induced a significant increase in the CD4/CD8 ratio in the lungs and lymph nodes (P < 0.001) and a significant decrease in the ratio in the spleen (P < 0.01). Furthermore, exposure to HBO induced a significant increase in T cells bearing surface interleukin-2 receptors in the blood, spleen, lungs, and lymph glands (P < 0.001) and a significant decrease in T cells expressing alpha beta-receptors in the lungs (P < 0.001) and lymph glands (P < 0.05). Our findings suggest rapid T-cell activation after a brief exposure to HBO, with shifts of CD4 and CD8 subsets and variations in T-cell receptor type. These rapid changes in the parameters of cell-mediated immunity may represent the activation of protective mechanisms against the toxic effect of oxygen or the early stages of pulmonary oxygen toxicity.

Animal Comparative Study CD4-CD8 Ratio *Hyperbaric Oxygenation Leukocyte Count Leukocytes, Mononuclear/*IMMUNOLOGY Lung/*IMMUNOLOGY Lymphoid Tissue/*IMMUNOLOGY Male Microscopy, Fluorescence Oxygen/*PHARMACOLOGY Rats Rats, Sprague-Dawley Support, Non-U.S. Gov't T-Lymphocyte Subsets/*IMMUNOLOGY JOURNAL ARTICLE



 




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