Natl Conf Hum Retroviruses Relat Infect (1st). 1993 Dec 12-16;:133.
Chronically and latently infected cell lines have been derived following
the acute infection of tissue culture cell lines with HIV-1. Although
cell lines of this type are often used to investigate the role of
inductive or inhibitory signals on HIV replication (U1, ACH-2, OM-10),
little is known about the biological characteristics of these cell
lines. We have biologically and biochemically evaluated chronically
infected CEM, MT2, H9 and U937 cell lines to characterize the infected
cells and compare them to the parental uninfected cells. In addition we
have examined the derivation of this population from the acutely
infected cells in terms of the virus infection. These studies were
performed by single cell cloning of the parental population and
virologic evaluation of the clones derived. Reverse transcriptase
activity assays and PCR were performed to determine that chronically
infected cells derived from cytopathically infected cell lines
predominantly included uninfected cells with a small subpopulation of
productively infected cells. In contrast, cell lines which were non-
cytopathically infected yielded a majority of productively infected
cells following acute infection. The clones derived from CEM and U937
cells responded differently to inductive signals such as TNF, IL6, and
PMA than did either the U1 or ACH-2 cells. Finally, the activity of
various antiviral agents was evaluated in our panel of chronically
infected lines. Several agents were found to have an effect on the
replication of HIV in these cultures. The mechanism of antiviral action
of these compounds will be presented.
*Drug Resistance, Microbial Microbial Sensitivity Tests RNA-Directed
DNA Polymerase/*ANTAGONISTS & INHIB ABSTRACT