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Persistence of unintegrated HIV-1 genomes in two human immature T cell lines.


Natl Conf Hum Retroviruses Relat Infect (1st). 1993 Dec 12-16;:125.

The human cell lines FT1 and FT4 have been obtained by cloning PBLs from an asymptomatic HIV-1 seropositive patient. They are IL-2-independent, form colonies in bactoagar, but are not tumorigenic in nude mice. FT cells are CD4- immature T cell lines and exhibit a silent unintegrated HIV-1 genome, since they do not express viral antigens nor shed viral particles. The two cell lines have been recloned and 6 clones (3 from each parental line) have been selected. The PCR analysis of discrete region of viral DNA (LTR, gag, pol, env) failed to show trivial deletions in the viral genome. Although the LTRs are present in the DNA of both parental and subcloned cells, preliminary studies show a polymorphism in their lengths that need to be evaluated by sequencing. The study of viral transcription by RNA-PCR in both parental and subcloned cell lines has shown total absence of transcription of structural (gag, env) and regulatory (rat, rev, nef) genes. The HIV genome is detectable in all cell lines in the cytoplasm when analyzed in confluent cultures. Work is in progress to determine whether the cytoplasm is the only localization of the viral genome or if it moves from nucleus to cytoplasm in exponentially growing cells.

Cell Line DNA, Viral Genome, Viral Human HIV-1/*GENETICS Polymerase Chain Reaction Sequence Deletion *Virus Integration ABSTRACT


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