Natl Conf Hum Retroviruses Relat Infect (1st). 1993 Dec 12-16;:125.
The human cell lines FT1 and FT4 have been obtained by cloning PBLs from
an asymptomatic HIV-1 seropositive patient. They are IL-2-independent,
form colonies in bactoagar, but are not tumorigenic in nude mice. FT
cells are CD4- immature T cell lines and exhibit a silent unintegrated
HIV-1 genome, since they do not express viral antigens nor shed viral
particles. The two cell lines have been recloned and 6 clones (3 from
each parental line) have been selected. The PCR analysis of discrete
region of viral DNA (LTR, gag, pol, env) failed to show trivial
deletions in the viral genome. Although the LTRs are present in the DNA
of both parental and subcloned cells, preliminary studies show a
polymorphism in their lengths that need to be evaluated by sequencing.
The study of viral transcription by RNA-PCR in both parental and
subcloned cell lines has shown total absence of transcription of
structural (gag, env) and regulatory (rat, rev, nef) genes. The HIV
genome is detectable in all cell lines in the cytoplasm when analyzed in
confluent cultures. Work is in progress to determine whether the
cytoplasm is the only localization of the viral genome or if it moves
from nucleus to cytoplasm in exponentially growing cells.
Cell Line DNA, Viral Genome, Viral Human HIV-1/*GENETICS Polymerase
Chain Reaction Sequence Deletion *Virus Integration ABSTRACT