Natl Conf Hum Retroviruses Relat Infect (1st). 1993 Dec 12-16;:125.
An Apparently healthy 5-year old male Macaca Fascicularis (Mf) was
experimentally infected i.v. with 10-20 MID50 of SIVmac251/32H. The
monkey died 3 months after infection. SIV genome was detected in PBMC by
PCR-DNA and the virus was rescued from PBMC cocultivated with CEMx174
cells. However, no detectable anti-SIV antibodies (by ELISA and RIPA
assays) were found in the plasma. Post-mortem histological examination
of tissues (lung, spleen, brain, lymph nodes) revealed a pattern
identical to that already described for SIV-infected monkeys in the late
phase to the disease. One month before death, the monkey developed a
rapidly growing retro-orbital mass which was histologically
characterized as a non-Hodgkin's large cell lymphoma.
Immunohistochemical analysis of the tumor mass revealed a reactivity to
CD45, CD4 and CD8, but not to CD20 and CD19 monoclonal antibodies, thus
suggesting a T cell origin of the tumor. Similar reactivity was detected
in lymph node tissues which were infiltrated by tumor cells.
Furthermore, no EBV genome was detected by in situ hybridization,
Southern blot and PCR- DNA. The presence of SIV genome in lymphoma
tissue was revealed both by PCR-DNA, using primers specific for gag and
env regions of SIV genome, and Southern blot. SIV was rescued from the
tumor by coculture with Cemx174 and Supt1 human cell lines.
Animal Antigens, CD/ANALYSIS Cell Line Enzyme-Linked Immunosorbent
Assay Immunohistochemistry Lymphoma, T-Cell/*COMPLICATIONS Macaca
fascicularis Male Monocytes/VIROLOGY Radioimmunoprecipitation Assay
Simian Acquired Immunodeficiency Syndrome/*COMPLICATIONS ABSTRACT