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Development of a T cell lymphoma in Macaca Fascicularis acutely infected with SIVmac251/32H.


Natl Conf Hum Retroviruses Relat Infect (1st). 1993 Dec 12-16;:125.

An Apparently healthy 5-year old male Macaca Fascicularis (Mf) was experimentally infected i.v. with 10-20 MID50 of SIVmac251/32H. The monkey died 3 months after infection. SIV genome was detected in PBMC by PCR-DNA and the virus was rescued from PBMC cocultivated with CEMx174 cells. However, no detectable anti-SIV antibodies (by ELISA and RIPA assays) were found in the plasma. Post-mortem histological examination of tissues (lung, spleen, brain, lymph nodes) revealed a pattern identical to that already described for SIV-infected monkeys in the late phase to the disease. One month before death, the monkey developed a rapidly growing retro-orbital mass which was histologically characterized as a non-Hodgkin's large cell lymphoma. Immunohistochemical analysis of the tumor mass revealed a reactivity to CD45, CD4 and CD8, but not to CD20 and CD19 monoclonal antibodies, thus suggesting a T cell origin of the tumor. Similar reactivity was detected in lymph node tissues which were infiltrated by tumor cells. Furthermore, no EBV genome was detected by in situ hybridization, Southern blot and PCR- DNA. The presence of SIV genome in lymphoma tissue was revealed both by PCR-DNA, using primers specific for gag and env regions of SIV genome, and Southern blot. SIV was rescued from the tumor by coculture with Cemx174 and Supt1 human cell lines.

Animal Antigens, CD/ANALYSIS Cell Line Enzyme-Linked Immunosorbent Assay Immunohistochemistry Lymphoma, T-Cell/*COMPLICATIONS Macaca fascicularis Male Monocytes/VIROLOGY Radioimmunoprecipitation Assay Simian Acquired Immunodeficiency Syndrome/*COMPLICATIONS ABSTRACT


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