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Proviral DNA from non producer interfering HIV-1-infected F12 cells abrogates HIVs susceptibility of He-La CD4+ cells.


Natl Conf Hum Retroviruses Relat Infect (1st). 1993 Dec 12-16;:125.

The Hut-78 F12 cell clone harbors a non producer HIV-1 variant and shows a complete resistance to HIV-1, HIV-2 or SIV superinfection. The replication of superinfecting HIV-2 is blocked during retrotranscription (Taddeo et al., Virology 1993). To establish if the expression of the F12- HIV variant was per sa necessary and sufficient to induce the homologous viral interference exhibited by F12 cells, the whole F12/HIV provirus was cloned and co-transfected in the He-La CD4+ cells with a plasmid carrying the Hygromycin B resistance. A number of He-La CD4+ cell clone were isolated and characterized. At variance with F12 cells, none of the clones expressing the F12/HIV genome were CD4-down- regulated as often observed in cells chronically infected with HIV; transfected clones were indistinguishably CD4+ (85-95%) as were He-La CD4+ cells. This notwithstanding, He- La CD4+ clones expressing the F12/HIV genome showed the same pattern of viral proteins as CD4- F12 cells. When they were challenged with HIV-1, replication of superinfecting HIV was either strongly inhibited or totally abolished, depending on the cell clone tested. F12 cells and a few F12/HIV-He-La CD4+ clones were transfected with the HIV infectious molecular clone pNL4-3. No release of infectious HIV was observed, indicating that the HIV life cycle is impaired in both types of cells during and following retrotranscription.

Clone Cells DNA, Viral/*PHYSIOLOGY Hela Cells Human HIV-1/*GENETICS/PHYSIOLOGY Proviruses/*GENETICS Transcription, Genetic Virus Replication ABSTRACT


Information in this article was accurate in December 30, 1995. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.