3rd Conf Retro and Opportun Infect. 1996 Jan 28-Feb 1;:165. Unique
Objectives: 1) To identify a serologic marker of infection with a novel
herpesvirus putatively associated with Kaposi's sarcoma (KS) and 2) to
determine the prevalence of KSHV infection as defined by serology in
HIV-infected patients with and without KS. Methods: KSHV antigens were
prepared from a cell-line, BC-1, provided by Moore and Chang. Immunoblot
and immunofluorescence assays were used to examine sera for evidence of
antibody production to viral antigens. Results: Treatment of BC-1 cells
with n-butyrate selectively triggered expression of new antigenic
KSHV-associated polypeptides that were recognized by sera from patients
with KS. Antibodies to a unique 40 kDa protein (p4O) from chemically
induced BC-1 cells were present in 67% of 48 KS patients compared to 13%
of 54 HIV-infected patients without KS (OR=13.43, 95% CI=4.48-42.0). The
positive predictive value of the serologic test for KS was 82%.
Imunofluorescence and immunoblot results were very closely correlated.
Conclusion: The presence of antibodies to an inducible antigen was
strongly correlated with clincally evident KS in HIV-infected subjects.
The serologic results support the hypothesis of an etiologic role for
Acquired Immunodeficiency Syndrome/COMPLICATIONS/*IMMUNOLOGY Antigens,
Viral/*BIOSYNTHESIS Butyrates/*PHARMACOLOGY Cell Line
Infections/*COMPLICATIONS/EPIDEMIOLOGY Human Prevalence Sarcoma,