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Assessment of maternal plasma HIV viral load as a correlate of vertical transmission.




 

3rd Conf Retro and Opportun Infect. 1996 Jan 28-Feb 1;:161. Unique

OBJECTIVE: To determine whether maternal plasma HIV viral load as measured by RT PCR for HIV RNA predicts vertical transmission. METHODS: Plasma was collected at 1st, 2nd, and 3rd trimester and delivery (visits 1,2,3 and 4, respec) from HIV-infected pregnant women enrolled to the Women and Infant Transmission Study at 6 sites in the US and Puerto Rico and stored at -7OC until thawed and analyzed in batch. HIV-1 RNA was determined using the Roche Amplicor HIV Monitor Kit (400 copies/ml dectable) after deheparinization by silica binding. All assays were run with the complete ACTG QC panel. HIV-infected offspring were classified as early if positive culture less than or equal to 7 d of age and late if neg 1st culture, then pos at greater than 7 d of age. Infants with neg cultures for greater than 6 mo were defined as uninfected. 115 maternal sample sets were analyzed (43ZDV tx). Results:(Table: See Text). In the ZDV naive qroup at delivery, 0/12 transmitted if less than 1000 copies/ml; 7/20 if 1,000-5000; 6/12 if 5,000-10,000; and 11/28 if greater than or equal to 10,0OO copies HIV RNA/ml respectively. CONCLUSIONS: ZDV naive transmitters tended to have higher RNA copy no. than non-transmitters. Mothers of infants with an early positive culture tended to have higher RNA copy no. than those of late positive infants. Lack of transmission was associated with a low/undetectable maternal RNA, but an absolute correlation with rate of transmission was not seen.

*Disease Transmission, Vertical Female HIV Infections/BLOOD/*TRANSMISSION HIV-1/GENETICS/*ISOLATION & PURIF Human Polymerase Chain Reaction Pregnancy Pregnancy Complications, Infectious ABSTRACT



 




Information in this article was accurate in November 30, 1996. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.