3rd Conf Retro and Opportun Infect. 1996 Jan 28-Feb 1;:157. Unique
Objective : To determine the relationship between cell-associated
infectious HIV-I virus and HIV-1 provirus with or without antiretroviral
therapy. Methods : 30 patients (CDC class A : n = 10, CDC class B : n =
8, CDC class C : n = 12 ; mean CD4 cell counts: 250/mm(3)) having
started antiretroviral therapy (AZT +/- ddl or ddC) were studied.
Peripheral blood samples were collected at W-2, W0 and W4. Quantitative
coculture using ANRS consensus technique was performed on fresh
specimens. HIV-1 proviral DNA level was assessed by end-point dilution
PCR using Amplicor TM (Roche) and a robotic workstation (Beckman).
Results : 1/ Natural variability (W-2 vs W0) was 0.5 log10 and 0.3 log10
for cell-associated infectious virus and proviral DNA respectively.
2/Baseline levels of cell-associated infectious virus and proviral DNA
were correlated (r=0.66, p=0.0001). 3/ One month after initiation of
therapy a 1.12 log10 decrease in infectious virus vs only a 0.24 log10
decrease in proviral DNA were observed. Conclusions : 1/ PBMC
quantitative culture and HIV-1 proviral DNA quantitation were well
correlated before treatment. 2/ After initiating therapy with nucleoside
analogs a marked decrease in cell-associated infectious virus contrasted
with little change in HIV-1 proviral DNA levels.
Cells, Cultured HIV Infections/BLOOD/*VIROLOGY
HIV-1/GENETICS/*ISOLATION & PURIF Human Monocytes/*VIROLOGY