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Assessment of viral burden using PBMC culture and HIV-1 proviral DNA quantitation.




 

3rd Conf Retro and Opportun Infect. 1996 Jan 28-Feb 1;:157. Unique

Objective : To determine the relationship between cell-associated infectious HIV-I virus and HIV-1 provirus with or without antiretroviral therapy. Methods : 30 patients (CDC class A : n = 10, CDC class B : n = 8, CDC class C : n = 12 ; mean CD4 cell counts: 250/mm(3)) having started antiretroviral therapy (AZT +/- ddl or ddC) were studied. Peripheral blood samples were collected at W-2, W0 and W4. Quantitative coculture using ANRS consensus technique was performed on fresh specimens. HIV-1 proviral DNA level was assessed by end-point dilution PCR using Amplicor TM (Roche) and a robotic workstation (Beckman). Results : 1/ Natural variability (W-2 vs W0) was 0.5 log10 and 0.3 log10 for cell-associated infectious virus and proviral DNA respectively. 2/Baseline levels of cell-associated infectious virus and proviral DNA were correlated (r=0.66, p=0.0001). 3/ One month after initiation of therapy a 1.12 log10 decrease in infectious virus vs only a 0.24 log10 decrease in proviral DNA were observed. Conclusions : 1/ PBMC quantitative culture and HIV-1 proviral DNA quantitation were well correlated before treatment. 2/ After initiating therapy with nucleoside analogs a marked decrease in cell-associated infectious virus contrasted with little change in HIV-1 proviral DNA levels.

Cells, Cultured HIV Infections/BLOOD/*VIROLOGY HIV-1/GENETICS/*ISOLATION & PURIF Human Monocytes/*VIROLOGY Proviruses/GENETICS ABSTRACT



 




Information in this article was accurate in November 30, 1996. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.