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Variation in estimates of HIV-1 plasma RNA concentration among laboratories and assay kits.


3rd Conf Retro and Opportun Infect. 1996 Jan 28-Feb 1;:155. Unique

The AIDS Clinical Trials Group (ACTG) has undertaken a preliminary evaluation of the variation in estimates of plasma HIV-1 RNA among laboratories and among three commercially available kits for the quantitation of HIV-1 plasma RNA (Roche - Amplicor; Chiron - bDNA 1.0; Organon Teknika - NASBA) . Laboratories were sent a common set of standards and a coded panel of HIV-1 spiked serum samples for proficiency testing. To be proficient a laboratory had to be able to detect five-fold differences in RNA concentration. This analysis is based on data from laboratories that were proficient. We have tentatively concluded the following: 1. Inter-laboratory variation for laboratories using the same kits can be reduced using a common set of standards, although there is still some variation among laboratories. , and 2. Absolute RNA copy number differences among kits was consistent, although there was a small difference between the RNA concentration estimates for the Amplicor and NASBA kits.

HIV-1/GENETICS/*ISOLATION & PURIF Human RNA, Viral/*BLOOD Reagent Kits, Diagnostic/*STANDARDS Reference Standards Reproducibility of Results ABSTRACT


Information in this article was accurate in November 30, 1996. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.