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Detection of circulating T cells with CD4+CD7- immunophenotype in patients with benign and malignant lymphoproliferative dermatoses.




 

J Am Acad Dermatol. 1996 Sep;35(3 Pt 1):404-10. Unique Identifier :

BACKGROUND: Cutaneous T-cell lymphomas (CTCLs) are malignancies of CD4+ T cells that involve the skin. CD4+CD7- cells may represent a malignant population in CTCL. OBJECTIVE: Our purpose was to compare the percentage of CD4+CD7- cells and the expression of pan-T-cell antigens in blood lymphocytes from 31 patients with benign dermatoses with 35 patients who had CTCL. METHODS: The patients with CTCL were classified as follows: 10 with mycosis fungoides (MF), seven with pre-Sezary syndrome (pre-SS), and 18 with Sezary syndrome (SS). Flow cytometry was used to determine the percentage of CD4+CD7- cells and the CD4/CD8 ratio and to detect aberrant expression of the pan-T-cell antigens CD2, CD3, and CD5. RESULTS: We found a mean of 5.8% CD4+CD7- cells for the 16 normal control subjects and 9.3% for the benign cases (p = 0.13). The patients with pre-SS and SS had a higher percentage of CD4+CD7- cells (22.4% and 35.5%, respectively) than patients with benign dermatoses (p < 0.01); no difference was found between patients with benign dermatoses and those with MF (p = 0.80). The mean CD4/CD8 ratio was 3.1 for the normal control subjects compared with 7.4 for the patients with benign dermatoses (p < 0.01). Patients with SS had a ratio of 49, which was higher than the ratio for those with benign dermatoses (p < 0.01); however, the ratio for patients with MF and pre-SS did not differ from that of the group with benign dermatoses (p = 0.71 and 0.55, respectively). Aberrant CD2, CD3, or CD5 expression was observed in 66% of patients with SS, 29% with pre-SS, 30% with MF, but in none of the patients with a benign dermatosis. CONCLUSION: Small numbers of CD4+CD7- cells can be found by flow cytometry in patients with a benign dermatosis and in normal control subjects. This T-cell subset is expanded in pre-SS and SS but not in MF. Aberrant pan-T-cell antigen expression is commonly observed in patients with SS but not in patients with a benign dermatosis.

Antigens, CD2/ANALYSIS Antigens, CD3/ANALYSIS Antigens, CD4/*ANALYSIS Antigens, CD5/ANALYSIS Antigens, CD7/*ANALYSIS Comparative Study CD4-CD8 Ratio Dermatitis, Atopic/BLOOD/IMMUNOLOGY Dermatitis, Contact/BLOOD/IMMUNOLOGY Dermatitis, Phototoxic/BLOOD/IMMUNOLOGY Flow Cytometry Human Immunophenotyping Lymphoma, T-Cell, Cutaneous/*BLOOD/IMMUNOLOGY Mycosis Fungoides/BLOOD/IMMUNOLOGY Neurodermatitis/BLOOD/IMMUNOLOGY Psoriasis/BLOOD/IMMUNOLOGY Sezary Syndrome/BLOOD/IMMUNOLOGY Skin/IMMUNOLOGY Skin Diseases/*BLOOD/IMMUNOLOGY Skin Neoplasms/*BLOOD/IMMUNOLOGY Support, Non-U.S. Gov't T-Lymphocyte Subsets/IMMUNOLOGY/*PATHOLOGY JOURNAL ARTICLE



 




Information in this article was accurate in December 30, 1996. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.