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Study of HIV-1/HIV-2 dual infection in Bombay.


Int Conf AIDS. 1996 Jul 7-12;11(1):169 (abstract no. Mo.C.1668). Unique

Objective: To estimate the prevalence of HIV-1 and HIV-2 in Bombay and investigate the interaction of HIV-1 and HIV-2 with respect to viral replication in HIV-1/HIV-2 dually infected patients. Methods:Blood samples were collected from 60 outpatients in G.T.Hospital, Bombay. Seroprevalence of HIV-1 and HIV-2 were investigated by the particle agglutination (PA) method and indirect immunofluorescence assay (IFA). HIV-1 and HIV-2 proviral DNA in primary peripheral blood mononuclear cells (PBMC) were detected by nested polymerase chain reaction (PCR). Quantitative competitive polymerase chain reaction (QC-PCR) method was used to quantify virion associated HIV-1 and HIV-2 RNA in plasma. Results: According to the results of PA, IFA and PCR, 60 Bombay patients were classified into five categories: 32 were HIV-1 single infection (53.3%), 15 were HIV-1/HIV-2 dual infection (25%), 3 were HIV-2 single infection (5%), 7 were HIV noninfection (11.7%) and 3 were indeterminate (5%). Although the mean HIV-1 viral load of HIV-1/HIV-2 dually infected group 79,330 copies/ml plasma) did not significantly different from that of HIV-1 single infected group (86,640 copies/ml plasma), but we noted the extremely separated distribution in HIV-1/HIV 2 dually infected group ( less than 6,000 copies/ml plasma in 6 patients and greater than 160,000 copies/ml plasma in 4 patients), compared with distribution of HIV-1 viral load in HIV-1 singly infected group. Despite the viral load of HIV-2 lower than that of HIV-1 in the same HIV-1/HIV-2 dually infected patients, we did not observe the significant difference of HIV-2 viral load between the HIV-1/HIV-2 dually infected group and HIV-2 single infected group.(This comparison may lack statistical power due to small number of HIV-2 single infected patients.)Conclusion: In Bombay, HIV-1 infection is predominant and HIV-1/HIV-2 dual infection is more common than HIV-2 single infection. In some dually infected patients, the existence of HIV-2 may have some inhibitory effect, on HIV-1 replication, but the order of HIV infection and subtype of HIV-2 may be crucial: whether HIV-1 can not replicate well in previously HIV-2 infected patients or whether only some subtype of HIV-2 inhibits HIV-1 replication require further study.



Information in this article was accurate in January 30, 1997. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.