Resource Logo
NLM AIDSLINE

Direct cytotoxicity of HIV-1 envelope protein gp120 on human NT neurons.




 

Neuroreport. 1996 Apr 10;7(5):1045-9. Unique Identifier : AIDSLINE

A new in vitro system comprising a pure population of neurons, human NT cells, was used to characterize the direct neurotoxic effect of HIV-1 envelope protein gp120. Cytotoxicity was monitored by a quantitative assay after exposure to recombinant gp120 in the presence or absence of other reagents. Treatment of mature NT neurons with various doses of gp120 for 24 h caused a decrease of up to 27% in the number of viable cells. This neurotoxicity was abolished by co-treatment with either D-2-amino-5-phosphonopentanoic acid (APV), MK801 or nimodipine, or by culturing cells in a Ca(2+)-free environment. Taken together, these data indicate that gp120 exerts a direct neurotoxic effect by acting through NMDA receptors and Ca2+ channels.

Dizocilpine Maleate/PHARMACOLOGY Excitatory Amino Acid Agonists/PHARMACOLOGY Human HIV Envelope Protein gp120/*TOXICITY *HIV-1 Neurons/*DRUG EFFECTS Neuroprotective Agents/PHARMACOLOGY Receptors, N-Methyl-D-Aspartate/AGONISTS Recombinant Proteins/TOXICITY Support, U.S. Gov't, P.H.S. Tumor Cells, Cultured 2-Amino-5-phosphonovalerate/PHARMACOLOGY JOURNAL ARTICLE



 




Information in this article was accurate in February 28, 1997. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.