a simplified procedure for the purification of trichosanthin (a type 1 ribosome inactivating protein) from trichosanthes kirilowii root tubers.

NLM AIDSLINE A simplified procedure for the purification of trichosanthin (a type 1 ribosome inactivating protein) from Trichosanthes kirilowii root tubers. Bhatia N; McDonald KA; Jackman AP; Dandekar AM; Department of Chemical
April 30, 1997

Protein Expr Purif. 1996 Mar;7(2):143-6. Unique Identifier : AIDSLINE A one step rapid and simple purification procedure for trichosanthin, a type 1 ribosome inactivating protein, from root tubers of Trichosanthes kirilowii has been developed using cation-exchange perfusion chromatography. The identity of the protein has been confirmed by its size, immunoreactivity, and sequence information. Yields of 0.16% of electrophoretically pure trichosanthin from dried root tuber have been achieved with a single 10-min chromatographic step giving the ability to purify gram quantities of trichosanthin in 1 day. Drugs, Chinese Herbal/ISOLATION & PURIF Trichosanthin/ISOLATION & PURIF

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