Mol Cell Probes. 1996 Dec;10(6):477-80. Unique Identifier : AIDSLINE
We describe a semiquantitative method to measure hepatitis C virus (HCV)
viral particle numbers, by carrying out reverse-transcription polymerase
chain reaction (RT-PCR) on serial dilutions of serum samples. The virus
concentrations measured were 10(3)-10(6) viral particles ml-1 of serum.
The method described is relatively quick, and the only required
manipulation is dilution of the serum. An optimal RT-PCR method is used
for diluted and undiluted samples.
*Hepatitis C/VIROLOGY *Hepatitis C-Like Viruses/ISOLATION & PURIF
*RNA, Viral/BLOOD *Viral Load/METHODS