Vopr Virusol. 1997 Sep-Oct;42(5):205-8. Unique Identifier : AIDSLINE
The polypeptide composition of HIV-I virus-like particles produced by
CV-I cells during mono- and coinfection with recombinant vaccinia virus
(rVV) strains containing the whole p55) and carboxyterminal truncated
(p48) gag genes and gag-pol sequence is studied. In monoinfection both
the gag-strains actively produced virus-like particles consisting of
non-processed p55Gag and p48Gag polyprotein without p6 domain. In case
of a coinfection of the cells with one of these strains and the rVV
producing p160Gag-Pol polyprotein the virus-like particles consisted of
p24 protein and a negligible amount of non-processed Gag precursors. The
share of p24 protein increased in proportion to the duration of
coinfection and decreased with a reduction of multiplicity of infection
with rVV carrying p160Gag-Pol. Hence, the absence of p6 domain does not
influence the processing of Gag proteins during virus-like particles
assembly and budding. In contrast to the natural systems of HIV-I
development, in the rVV expression system the p6Gag domain virtually
does not contribute to reactions between Gag and Gag-Pol precursors and
to the particles' morphogenesis.
*Fusion Proteins, gag-pol/METABOLISM *Gene Products, gag/METABOLISM
*HIV-1/METABOLISM *Protein Precursors/METABOLISM *Vaccinia
Virus/GENETICS *Virion/METABOLISM