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Interaction of HIV-1 Tat with the p300/CBP transcriptional co-activators.




 

HIV Pathog Treat Conf. 1998 Mar 13-19;:45 (abstract no. 1027). Unique

The human immunodeficiency virus encodes the Tat transactivator protein that is essential for viral replication and progression to disease. Here we demonstrate that transcriptional activation by HIV-1 Tat involves p300 or the related cellular transcriptional coactivator, CREB binding protein (CBP). Tat transactivation was inhibited by the 12S form of the adenoviral EIA gene product which inhibits p300 function, and this inhibition was independent of its effect on NF-kappaB transcription. A biochemical interaction of p300 with Tat was demonstrated in vitro and in vivo by co-immunoprecipitation. The carboxy-terminal region of p300, which binds to EIA, was shown to bind specifically to the highly conserved basic domain of Tat which also mediates binding to the TAR RNA stem loop structure. The ability of Tat to interact physically and functionally with this coactivator provides a mechanism to link Tat to the basal transcription complex.

MEETING ABSTRACTS Acetyltransferases/*METABOLISM Cell Cycle Proteins/*METABOLISM Gene Products, tat/*METABOLISM HIV-1/*METABOLISM Nucleic Acid Conformation Precipitin Tests Protein Binding RNA, Viral/CHEMISTRY/METABOLISM Trans-Activators/*METABOLISM



 




Information in this article was accurate in December 30, 1998. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.