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Detection of HIV-1 infection by immunologycal and molecular methods in seronegative women, sexual partners of HIV-1 infected individuals.


Int Conf AIDS. 1998;12:1097 (abstract no. 60529). Unique Identifier :

OBJECTIVES: Evidences of HIV-1 infection have been described in seronegative subjects belonging to high risk groups. In this sense, the aim of this study was to investigate the possibility of early detection of HIV-1 infection and the presence of seroconvertion markers in HIV-1 seronegative women, sexual partners of seropositive individuals and AIDS patients, participating in a multicenter study of heterosexual transmission of HIV in Rio de Janeiro. METHODOLOGY: Serologically negative or indeterminate (IND) women (222) were selected from this study. Anti-HIV-1 antibodies were evaluated by ELISA (Abbott HIV-1-recombinant EIA, USA), indirect immunofluorescence (IFI), using slides with HIV-1 infected K-37 cell lines (Biomanguinhos/FIOCRUZ, BR) and confirmed by Western blot (WB). Nested PCR detection for env, gag and pol regions of HIV-1 proviral DNA and p24 viral antigen (HIV-1 p24 Ag. ELISA, DuPont, USA) and co-culture methods were performed to assess the presence of viral infection. In vitro antibody spontaneously produced (IVAP) was also assessed. Moreover, a follow up of 102 seronegative women belonging to this group was also performed in order to evidenciate a possible seroconversion. RESULTS: 34 out of 222 seronegative women (15.31%) showed INDWB and one of the 18 that returned during the follow up, seroconverted. No seroconvertion was observed in those presenting IND/positive reactions by ELISA, IFI and IVAP. On the other hand, 15 of 114 samples, evaluated for p24 viral Ag detection, were positive, and 2 seroconverted. 4 seronegative women were PCR positive and virus was isolated in 3 of these samples however, no seroconversion was observed during the follow-up. 4 from the 102 women followed in this study seroconverted along the follow-up, however only one of them was negative in all tests in her previous blood sample collection. CONCLUSION: PCR amplified proviral DNA, virus isolation and p24 antigen detection in some seronegative samples indicate the possibility of HIV-1 infection and viral replication. The presence of HIV-1 antibodies in IND reactions could be suggestive of exposition the HIV-1 proteins or virus particles, probably during sexual intercourse. The absence of seroconvertion in these women gives support to the possibility of long lasting immunological window period and/or transient HIV-1 infection.

MEETING ABSTRACTS Brazil Contact Tracing Enzyme-Linked Immunosorbent Assay Female Human HIV Antibodies/ANALYSIS HIV Infections/*DIAGNOSIS/TRANSMISSION *HIV Seronegativity *HIV-1/ISOLATION & PURIF Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S.


Information in this article was accurate in December 30, 1998. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.