Resource Logo
NLM AIDSLINE

Inhibitory effect of biphalin and AZT on murine Friend leukemia virus infection in vitro.




 

Int J Immunopharmacol. 1998 Sep;20(9):457-66. Unique Identifier :

Biphalin is a bivalent opioid analogue containing two tyrosine residues. We have examined the effect of biphalin's anti-retroviral potency in vitro using a murine model. Biphalin, in non-cytotoxic concentrations, suppressed in a dose-dependent fashion the replication of Friend leukemia virus (FLV) in Mus dunni cells as determined using a focus forming assay. FLV replication was substantially reduced by biphalin at 10(-4) M concentration. When biphalin was combined with 3'-azido-3'-deoxythymidine (AZT) the two acted synergistically in inhibiting FLV replication compared to either used alone. Using a reverse transcriptase (RT) assay, FLV RT levels also were noted to be reduced in the presence of biphalin. These observations indicate that biphalin possesses anti-retroviral activity in vitro, suggesting that this opioid peptide should be examined further in vivo to determine if it is a candidate for combined therapy with AZT and possibly other drugs for retrovirus infections including the human immunodeficiency virus (HIV).

JOURNAL ARTICLE Antiviral Agents/*PHARMACOLOGY Cell Line Dose-Response Relationship, Drug Enkephalins/*PHARMACOLOGY Friend Virus/*DRUG EFFECTS/PHYSIOLOGY Human RNA-Directed DNA Polymerase/METABOLISM Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Virus Replication/DRUG EFFECTS Zidovudine/*PHARMACOLOGY



 




Information in this article was accurate in March 30, 1999. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.