protection against parenteral hiv-1 infection by homozygous deletion in the c-c chemokine receptor 5 gene.

NLM AIDSLINE Protection against parenteral HIV-1 infection by homozygous deletion in the C-C chemokine receptor 5 gene. Kupfer B; Kaiser R; Brackmann HH; Effenberger W; Rockstroh JK; Matz B;
November 30, 1999

AIDS. 1999 Jun 18;13(9):1025-8. Unique Identifier : AIDSLINE OBJECTIVES: To investigate the role of the CC chemokine receptor 5 (CCR5) for parenteral transmission of HIV-1. DESIGN: The prevalence of the delta32 deletion within the CCR5 gene was determined in a cohort of 207 patients, who had received documented amounts of non-antibody-tested and non-inactivated clotting factor concentrate. METHODS: Chromosomal DNA of haemophiliacs was isolated from whole blood. A portion of the CCR5 gene spanning the delta32 deletion was amplified by PCR. The resulting DNA fragments were analysed by agarose gel electrophoresis. RESULTS: The rate of HIV-1 infection was correlated strongly with increasing amounts of inoculated clotting factor concentrate. None of the HIV-positive patients (n = 129) had the delta32/delta32 genotype, whereas 12 out of 78 HIV-negative haemophiliacs had the homozygous delta32 deletion. CONCLUSIONS: The delta32/delta32 genotype was highly protective against HIV-1 infection, even in patients who had received millions of non-inactivated clotting factor units. As it is likely that in the early 1980s plasma pools were contaminated not only with monocyte-tropic HIV-1 strains, CCR5 appears to be the major mediator of HIV-1 infection. Furthermore, we conclude that there must be other protective mechanisms in multiply exposed non-infected haemophiliacs who have wild-type CCR5. JOURNAL ARTICLE Base Sequence Cohort Studies CD4 Lymphocyte Count DNA/ANALYSIS Genotype Hemophilia A/COMPLICATIONS/GENETICS Human HIV Infections/COMPLICATIONS/IMMUNOLOGY/TRANSMISSION HIV-1/PHYSIOLOGY Receptors, CCR5/*GENETICS RNA, Viral/BLOOD Sequence Deletion

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Information in this article was accurate in November 30, 1999. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.