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Cutting edge: activation of NK T cells by CD1d and alpha-galactosylceramide directs conventional T cells to the acquisition of a Th2 phenotype.


J Immunol. 1999 Sep 1;163(5):2373-7. Unique Identifier : AIDSLINE

NK T cells recognize glycolipid Ags such as alpha-galactosylceramide (alpha-GalCer) presented by the MHC class I-like molecule CD1d. In this paper we have studied the in vivo effects of alpha-GalCer on the generation of adaptive immune responses. Treatment of mice with alpha-GalCer resulted in rapid activation of NK T cells and production of the cytokines IL-4 and IFN-gamma. However, after this initial stimulation, NK T cells became polarized for the production of IL-4. Further, as soon as 6 days after alpha-GalCer injection, a marked increase in serum IgE levels was observed. Administration of alpha-GalCer at the time of priming of mice with protein Ag resulted in the generation of Ag-specific Th2 cells and a profound increase in the production of IgE. Collectively, these findings indicate that alpha-GalCer may be useful for modulating immune responses toward a Th2 phenotype during prophylaxis and therapy.

JOURNAL ARTICLE Adjuvants, Immunologic/ADMINISTRATION & DOSAGE Animal Antigens, CD1/GENETICS/*IMMUNOLOGY B-Lymphocytes/IMMUNOLOGY Cell Differentiation/IMMUNOLOGY Cytokines/BIOSYNTHESIS Galactosylceramides/ADMINISTRATION & DOSAGE/*IMMUNOLOGY Immunity, Cellular/IMMUNOLOGY Immunophenotyping Injections, Intraperitoneal Killer Cells, Natural/*IMMUNOLOGY/METABOLISM Lymphocyte Transformation/*IMMUNOLOGY Mice Mice, Inbred C57BL Mice, Mutant Strains T-Lymphocyte Subsets/*IMMUNOLOGY/METABOLISM Th1 Cells/METABOLISM Th2 Cells/IMMUNOLOGY/*METABOLISM


Information in this article was accurate in November 30, 1999. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.