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NLM AIDSLINE

A V3 loop haptenic peptide sequence, when tandemly repeated, enhances immunogenicity by facilitating helper T-cell responses to a covalently linked carrier protein.




 

Vaccine. 1999 May 14;17(19):2392-9. Unique Identifier : AIDSLINE

Subunit immunogens containing tandemly repeated copies of T- and B-cell epitopes have been shown to be more immunogenic than the respective immunogen containing only a single copy of the sequence. It has been unclear, however, whether the increased immunogenicity of a tandemly repeated B-cell epitope necessarily results from increased helper T-cell responses to intrinsic T-cell epitopes in the tandemly repeated sequences, or to neodeterminant T-cell epitopes created at the junction of adjacent repeat sequences. We examined this question by comparing the immunogenicity in mice of two immunogens containing one or eight tandemly repeated copies of an HIV-1 V3 loop haptenic sequence. Our results show that the tandemly repeated haptenic sequence potentiates the immunogenicity of the protein construct, likely through the facilitation of enhanced B-cell interaction with the tandem repeat construct and the consequent elicitation of increased carrier protein-specific helper T-cell responses.

JOURNAL ARTICLE Amino Acid Sequence Animal B-Lymphocytes/IMMUNOLOGY Carrier Proteins/*IMMUNOLOGY H-2 Antigens/IMMUNOLOGY/METABOLISM Haptens/*IMMUNOLOGY HIV Antibodies/BIOSYNTHESIS HIV Envelope Protein gp160/*IMMUNOLOGY HIV Infections/IMMUNOLOGY/PREVENTION & CONTROL HIV-1/CHEMISTRY/IMMUNOLOGY ISCOMs/*IMMUNOLOGY Male Mice Mice, Inbred BALB C Mice, Nude Molecular Sequence Data Peptides/*IMMUNOLOGY Repetitive Sequences, Amino Acid Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. T-Lymphocytes, Helper-Inducer/*IMMUNOLOGY



 




Information in this article was accurate in November 30, 1999. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.