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Alpha(1,3)-fucosyltransferase VII and alpha(2,3)-sialyltransferase IV are up-regulated in activated CD4 T cells and maintained after their differentiation into Th1 and migration into inflammatory sites.


J Immunol. 1999 Oct 1;163(7):3746-52. Unique Identifier : AIDSLINE

Activated Th1 CD4 T cells bind to P-selectin and migrate into inflamed tissue, whereas Th2 cells do not. We show that alpha(1, 3)-fucosyltransferase VII (FucT-VII) and alpha(2, 3)-sialyltransferase IV (ST3GalIV), which are crucial for the biosynthesis of functional P-selectin ligands, are absent in naive CD4 T cells, but are rapidly up-regulated upon activation. Th1 or Th2 differentiation in the presence of polarizing cytokines leads to down-regulation of FucT-VII mRNA selectively in Th2 but not in Th1 cells. Influencing the differentiation by varying the priming dose of antigenic peptide results in similar FucT-VII down-regulation only in Ag-specific Th2 cells. ST3GalIV levels remain elevated. FucT-VII and ST3GalIV mRNAs are also up-regulated by Th1 cells primed in vivo and recruited into the lymph nodes draining delayed-type hypersensitivity sites. We identify FucT-VII gene expression as a principal difference between Th1 and Th2 cells, and underscore the importance of FucT-VII and ST3GalIV expression for the biosynthesis of functional selectin ligands.

JOURNAL ARTICLE Animal Cell Differentiation/IMMUNOLOGY Cell Movement/*IMMUNOLOGY CD4-Positive T-Lymphocytes/*ENZYMOLOGY/IMMUNOLOGY/PATHOLOGY Down-Regulation (Physiology)/IMMUNOLOGY Epitopes, T-Lymphocyte/BIOSYNTHESIS Fucosyltransferases/*BIOSYNTHESIS/GENETICS Gangliosides/IMMUNOLOGY Histocompatibility Antigens Class II/METABOLISM Hypersensitivity, Delayed/ENZYMOLOGY/IMMUNOLOGY/PATHOLOGY Interleukin-12/PHARMACOLOGY Interleukin-4/PHARMACOLOGY Interphase/IMMUNOLOGY Lewis Blood-Group System/IMMUNOLOGY Lymph Nodes/ENZYMOLOGY/IMMUNOLOGY/*PATHOLOGY *Lymphocyte Transformation Mice Mice, Inbred BALB C Mice, Inbred C3H Mice, Inbred C57BL Mice, Transgenic Peptide Fragments/IMMUNOLOGY/METABOLISM RNA, Messenger/BIOSYNTHESIS Sialyltransferases/*BIOSYNTHESIS Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Th1 Cells/*ENZYMOLOGY/IMMUNOLOGY/PATHOLOGY Th2 Cells/ENZYMOLOGY/IMMUNOLOGY Up-Regulation (Physiology)/IMMUNOLOGY


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