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Alpha(1,3)-fucosyltransferase VII and alpha(2,3)-sialyltransferase IV are up-regulated in activated CD4 T cells and maintained after their differentiation into Th1 and migration into inflammatory sites.




 

J Immunol. 1999 Oct 1;163(7):3746-52. Unique Identifier : AIDSLINE

Activated Th1 CD4 T cells bind to P-selectin and migrate into inflamed tissue, whereas Th2 cells do not. We show that alpha(1, 3)-fucosyltransferase VII (FucT-VII) and alpha(2, 3)-sialyltransferase IV (ST3GalIV), which are crucial for the biosynthesis of functional P-selectin ligands, are absent in naive CD4 T cells, but are rapidly up-regulated upon activation. Th1 or Th2 differentiation in the presence of polarizing cytokines leads to down-regulation of FucT-VII mRNA selectively in Th2 but not in Th1 cells. Influencing the differentiation by varying the priming dose of antigenic peptide results in similar FucT-VII down-regulation only in Ag-specific Th2 cells. ST3GalIV levels remain elevated. FucT-VII and ST3GalIV mRNAs are also up-regulated by Th1 cells primed in vivo and recruited into the lymph nodes draining delayed-type hypersensitivity sites. We identify FucT-VII gene expression as a principal difference between Th1 and Th2 cells, and underscore the importance of FucT-VII and ST3GalIV expression for the biosynthesis of functional selectin ligands.

JOURNAL ARTICLE Animal Cell Differentiation/IMMUNOLOGY Cell Movement/*IMMUNOLOGY CD4-Positive T-Lymphocytes/*ENZYMOLOGY/IMMUNOLOGY/PATHOLOGY Down-Regulation (Physiology)/IMMUNOLOGY Epitopes, T-Lymphocyte/BIOSYNTHESIS Fucosyltransferases/*BIOSYNTHESIS/GENETICS Gangliosides/IMMUNOLOGY Histocompatibility Antigens Class II/METABOLISM Hypersensitivity, Delayed/ENZYMOLOGY/IMMUNOLOGY/PATHOLOGY Interleukin-12/PHARMACOLOGY Interleukin-4/PHARMACOLOGY Interphase/IMMUNOLOGY Lewis Blood-Group System/IMMUNOLOGY Lymph Nodes/ENZYMOLOGY/IMMUNOLOGY/*PATHOLOGY *Lymphocyte Transformation Mice Mice, Inbred BALB C Mice, Inbred C3H Mice, Inbred C57BL Mice, Transgenic Peptide Fragments/IMMUNOLOGY/METABOLISM RNA, Messenger/BIOSYNTHESIS Sialyltransferases/*BIOSYNTHESIS Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Th1 Cells/*ENZYMOLOGY/IMMUNOLOGY/PATHOLOGY Th2 Cells/ENZYMOLOGY/IMMUNOLOGY Up-Regulation (Physiology)/IMMUNOLOGY



 




Information in this article was accurate in December 30, 1999. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.