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NLM AIDSLINE

Compartmentalization of the IgG immune response to HIV-1 in breast milk.




 

AIDS. 1999 Jul 30;13(11):1323-31. Unique Identifier : AIDSLINE

OBJECTIVE: To evaluate the IgG immune response to HIV-1 in colostrum. METHODS: Paired serum and colostrum were collected from 16 asymptomatic HIV-1-infected women. IgG to gp160 and to four peptides (gp41 immunodominant DI domain, gp41/Id; EDLKWA epitope of DIII domain, gp41/K; gp120 C-terminus, gp120/Ct; V3 loop, gp120/V3) were evaluated in all samples. Functional activity of purified IgG was assessed for the ability to block transcytosis of cell-associated HIV-1 through a tight monolayer of endometrial epithelial cell line HEC1. RESULTS: IgG antibody to gp160 and to the four env-encoded synthetic peptides were detected in all specimens. The mean specific activity of IgG to gp41/K was 4.2 fold higher in colostrum than in paired serum. In contrast, mean specific activities of IgG to gp160 and gp41/Id were twofold higher in serum than in paired colostrum. Mean specific activities of IgG to gp120/V3 and to gp120/Ct were similar in systemic and milk compartments. Functional activity of IgG was evaluated in six paired serum and colostrum: in two women, serum IgG was 3.0 and 7.6 fold more efficient in blocking transcytosis than colostrum IgG; in one patient, colostrum IgG exhibited a 28 fold higher inhibitory capacity than serum IgG; in the remaining patients, serum and colostrum IgG demonstrated similar inhibitory activities against transcytosis of HIV. CONCLUSION: These features are consistent with a compartmentalization of the humoral IgG immune response to HIV within the mammary gland. Some HIV-1 antigens are able to induce a strong humoral mucosal immune response which may be of relevance for the design of a mucosal vaccine against HIV-1.

JOURNAL ARTICLE Adolescence Adult Animal Breast Feeding Cells, Cultured Colostrum/IMMUNOLOGY Endocytosis/DRUG EFFECTS Epitopes Female Human HIV Antibodies/*ANALYSIS/BLOOD HIV Antigens/IMMUNOLOGY HIV Envelope Protein gp120/CHEMISTRY/IMMUNOLOGY HIV Envelope Protein gp160/IMMUNOLOGY HIV Envelope Protein gp41/IMMUNOLOGY HIV Infections/*IMMUNOLOGY HIV-1/*IMMUNOLOGY/PHYSIOLOGY IgG/*ANALYSIS/BLOOD/IMMUNOLOGY Immunity, Mucosal Milk, Human/*IMMUNOLOGY Peptide Fragments/CHEMISTRY/IMMUNOLOGY Pregnancy Pregnancy Complications, Infectious/*IMMUNOLOGY/VIROLOGY Support, Non-U.S. Gov't



 




Information in this article was accurate in December 30, 1999. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.