FEBS Lett. 1999 Sep 17;458(2):151-6. Unique Identifier : AIDSLINE
Nine different DNA enzymes (DzV3-n, n=1-9) targeting the V3 loop region
of HIV-1 HXB2 were synthesized. One of those, DzV3-9, efficiently
cleaved the target in the conserved sequence in the RNA transcript in
vitro. DzV3-9 was stable in the cells and inhibited replication of both
NL432 and SF162 strains in U87 cells expressing CD4 and co-receptors.
The inhibitory effect of DNAzyme on incoming HIV-1 was also demonstrated
with pseudotype virions generated by NL432-based luciferase reporter
genes. Thus, an efficient, stable DNAzyme against a functionally
important region of HIV-1 was identified, and it may be useful for
prevention of HIV-1 infection.
JOURNAL ARTICLE Antiviral Agents/CHEMICAL SYNTHESIS/*PHARMACOLOGY Base
Sequence Catalysis Cell Line DNA, Single-Stranded/CHEMICAL
SYNTHESIS/*PHARMACOLOGY DNA, Viral/CHEMICAL SYNTHESIS/*PHARMACOLOGY
Enzyme Stability Gene Products, env/METABOLISM Human Hydrolysis HIV
Infections/ENZYMOLOGY/PREVENTION & CONTROL/VIROLOGY HIV-1/*DRUG
EFFECTS/PHYSIOLOGY/PATHOGENICITY Kinetics Molecular Sequence Data
Peptide Fragments/METABOLISM RNA, Viral/*METABOLISM Support, Non-U.S.
Gov't Virus Replication