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NLM AIDSLINE

Human neutralizing human immunodeficiency virus type 2-specific Fab molecules generated by phage display.




 

J Gen Virol. 1999 Aug;80 ( Pt 8):1987-93. Unique Identifier : AIDSLINE

A panel of human immunodeficiency virus type 2 (HIV-2)-neutralizing, recombinant Fab fragments was generated by using the phage display technique. The combinatorial library was derived from an asymptomatic, HIV-2-seropositive individual and constructed on the surface of filamentous phage by using the pComb3 phagemid vector and then screened against native HIV-2 envelope glycoprotein (gp125). Ten of 30 Fab fragments generated displayed strong reactivity in an ELISA and were therefore selected for further study. Six of these possessed neutralizing capacity, with titres varying from 20 to 80 against the homologous HIV-2 strain, and one also had a weak neutralizing capacity against a heterologous HIV-2 isolate, K135. Sequencing of the heavy chain CDR3 regions showed that the gp125-specific Fabs represented individual clones. These reagents may be useful for studies on the conformational structures of the HIV-2 envelope antigens and their immunogenicity, which may help in vaccine design. Furthermore, the cloned Fab genes may be transformed into whole IgG for eukaryotic expression, and as such used for therapeutic and immunoprophylactic studies in HIV-2-infected macaques and, possibly, for human immunoprophylaxis against HIV-2.

JOURNAL ARTICLE Amino Acid Sequence Antibody Specificity Bacteriophages Enzyme-Linked Immunosorbent Assay Gene Products, env/*IMMUNOLOGY Human HIV Antibodies/CHEMISTRY/*IMMUNOLOGY HIV Antigens/*IMMUNOLOGY HIV Infections/BLOOD/VIROLOGY HIV-2/*IMMUNOLOGY Immunoglobulins, Fab/CHEMISTRY/*IMMUNOLOGY Immunoglobulins, Heavy-Chain/IMMUNOLOGY Male Middle Age Molecular Sequence Data Neutralization Tests Peptide Library Protein Precursors/*IMMUNOLOGY Support, Non-U.S. Gov't



 




Information in this article was accurate in December 30, 1999. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.