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Manganese cations increase the mutation rate of human immunodeficiency virus type 1 ex vivo.


J Gen Virol. 1999 Aug;80 ( Pt 8):1983-6. Unique Identifier : AIDSLINE

Human immunodeficiency virus (HIV) reverse transcription is an error-prone process with an overall mutation rate of approximately 3.4 x 10(-5) per base per replication cycle. This rate can be modulated by changes in different components of the retrotranscription reaction. In particular, in vitro substitution of magnesium cations (Mg2+) by manganese cations (Mn2+) has been shown to increase misincorporation of deoxynucleotide triphosphates (dNTPs) and to alter substrate specificity. Here, it is shown that Mn2+ also increases the HIV mutation rate ex vivo. Treatment of permissive cells with Mn2+ and subsequent HIV infection resulted in at least 6-fold and 10-fold increases in the mutant and mutation frequencies respectively, thus illustrating a further example of how to influence HIV genetic variation.

JOURNAL ARTICLE Cations, Divalent Chlorides/PHARMACOLOGY Human HIV-1/DRUG EFFECTS/*GENETICS/PHYSIOLOGY HIV-1 Reverse Transcriptase/*GENETICS Manganese/*PHARMACOLOGY Manganese Compounds/PHARMACOLOGY *Mutagenesis Support, Non-U.S. Gov't Transcription, Genetic/DRUG EFFECTS U937 Cells


Information in this article was accurate in December 30, 1999. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.