Resource Logo

Identification of an MHC class I-restricted autoantigen in type 1 diabetes by screening an organ-specific cDNA library [see comments]


Nat Med. 1999 Sep;5(9):1026-31. Unique Identifier : AIDSLINE

Type 1 diabetes is an autoimmune disease in which the insulin-producing pancreatic beta cells are destroyed at an early age by an immune process that involves both CD4 and CD8 T lymphocytes. The identification of autoantigens in diabetes is very important for the design of antigen-specific immunotherapy. By screening a pancreatic islet cDNA library, we have identified the autoantigen recognized by highly pathogenic CD8 T cells in the non-obese diabetic mouse, one of the best animal models for human diabetes. This is the first identification, to our knowledge, of a CD8 T-cell epitope in an autoimmune disease. The peptide recognized by the cells is in the same region of the insulin B chain as the epitope recognized by previously isolated pathogenic CD4 T cells. This has very important implications for the potential use of insulin in preventative therapy.

JOURNAL ARTICLE Amino Acid Sequence Animal Autoantigens/*IMMUNOLOGY Clone Cells/IMMUNOLOGY/PATHOLOGY Cloning, Molecular CD8-Positive T-Lymphocytes/*IMMUNOLOGY/PATHOLOGY COS Cells Diabetes Mellitus, Insulin-Dependent/GENETICS/*IMMUNOLOGY Epitopes, T-Lymphocyte/CHEMISTRY/GENETICS/IMMUNOLOGY *Gene Library Histocompatibility Antigens Class I/*IMMUNOLOGY Insulin/CHEMISTRY/GENETICS/IMMUNOLOGY Interferon Type II/BIOSYNTHESIS Islets of Langerhans/*IMMUNOLOGY/METABOLISM/PATHOLOGY Lymphocyte Count Lymphocyte Transformation Mice Mice, Inbred NOD Mice, Inbred Strains Organ Specificity Peptides/CHEMISTRY/GENETICS/IMMUNOLOGY Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S.


Information in this article was accurate in December 30, 1999. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.