2012 SEP 17 (NewsRx) -- By a News Reporter-Staff News Editor at AIDS Weekly -- Investigators publish new report on AIDS/HIV. According to news reporting out of Los Alamos, New Mexico, by NewsRx editors, research stated, "Early diagnosis of active tuberculosis (TB) remains an elusive challenge, especially in individuals with disseminated TB and HIV co-infection. Recent studies have shown a promise for the direct detection of pathogen-specific biomarkers such as lipoarabinomannan (LAM) for the diagnosis of TB in HIV-positive individuals."
Our news journalists obtained a quote from the research from Los Alamos National Laboratory, "Currently, traditional immunoassay platforms that suffer from poor sensitivity and high non-specific interactions are used for the detection of such biomarkers. In this manuscript, we demonstrate the development of sandwich immunoassays for the direct detection of three TB-specific biomarkers, namely LAM, early secretory antigenic target 6 (ESAT6) and antigen 85 complex (Ag85), using a waveguide-based optical biosensor platform. Combining detection within the evanescent field of a planar optical waveguide with functional surfaces that reduce non-specific interactions allows for the ultra-sensitive and quantitative detection of biomarkers (an order of magnitude enhanced sensitivity, as compared to plate-based ELISA) in complex patient samples (urine, serum) within a short time. We also demonstrate the detection of LAM in urine from a small sample of subjects being treated for TB using this approach with excellent sensitivity and 100% corroboration with disease status. These results suggest that pathogen-specific biomarkers can be applied for the rapid and effective diagnosis of disease."
According to the news editors, the researchers concluded: "It is likely that detection of a combination of biomarkers offers greater reliability of diagnosis, rather than detection of any single pathogen biomarker."
For more information on this research see: Rapid detection of Mycobacterium tuberculosis biomarkers in a sandwich immunoassay format using a waveguide-based optical biosensor. Tuberculosis, 2012;92(5):407-16. (Elsevier - www.elsevier.com; Tuberculosis - www.elsevier.com/wps/product/cws_home/638428)
Our news journalists report that additional information may be obtained by contacting H. Mukundan, Chemistry Division, Los Alamos National Laboratory, Los Alamos, NM 87545, United States (see also AIDS/HIV).
Keywords for this news article include: AIDS/HIV, Los Alamos, New Mexico, Biosensing, United States, Actinobacteria, Bioengineering, Mycobacteriaceae, Bionanotechnology, Nanobiotechnology, Gram Positive Rods, Infectious Disease, Mycobacterium Infections, North and Central America, Actinomycetales Infections, Mycobacterium Tuberculosis.
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