2014 JAN 13 (NewsRx) -- By a News Reporter-Staff News Editor at AIDS Weekly -- New research on Immune System Diseases and Conditions is the subject of a report. According to news reporting originating in Baltimore, Maryland, by NewsRx journalists, research stated, "SAMHD1 is a dGTP-activated deoxynucleoside triphosphate triphosphohydrolase (dNTPase) whose dNTPase activity has been linked to HIV/SIV restriction. The mechanism of its dGTP-activated dNTPase function remains unclear."
The news reporters obtained a quote from the research from the Johns Hopkins University Bloomberg School of Public Health, "Recent data also indicate that SAMHD1 regulates retrotransposition of LINE-1 elements. Here we report the 1.8-angstrom crystal structure of homotetrameric SAMHD1 in complex with the allosteric activator and substrate dGTP/dATP. The structure indicates the mechanism of dGTP-dependent tetramer formation, which requires the cooperation of three subunits and two dGTP/dATP molecules at each allosteric site. Allosteric dGTP binding induces conformational changes at the active site, allowing a more stable interaction with the substrate and explaining the dGTP-induced SAMHD1 dNTPase activity. Mutations of dGTP binding residues in the allosteric site affect tetramer formation, dNTPase activity and HIV-1 restriction. dGTP-triggered tetramer formation is also important for SAMHD1-mediated LINE-1 regulation."
According to the news reporters, the research concluded: "The structural and functional information provided here should facilitate future investigation of SAMHD1 function, including dNTPase activity, LINE-1 modulation and HIV-1 restriction."
For more information on this research see: Structural insight into dGTP-dependent activation of tetrameric SAMHD1 deoxynucleoside triphosphate triphosphohydrolase. Nature Communications, 2013;4():20-28. Nature Communications can be contacted at: Nature Publishing Group, Macmillan Building, 4 Crinan St, London N1 9XW, England. (Nature Publishing Group - www.nature.com/; Nature Communications - www.nature.com/ncomms/)
Our news correspondents report that additional information may be obtained by contacting C.F. Zhu, Johns Hopkins Bloomberg Sch Public Hlth, Dept. of Mol Microbiol & Immunol, Baltimore, MD 21205, United States. Additional authors for this research include W.Y. Gao, K. Zhao, X.H. Qin, Y.J. Zhang, X. Peng, L. Zhang, Y.H. Dong, W.Y. Zhang, P. Li, W. Wei, Y. Gong and X.F. Yu (see also Immune System Diseases and Conditions).
Keywords for this news article include: Maryland, Genetics, HIV/AIDS, Baltimore, RNA Viruses, Retroviridae, United States, HIV Infections, Vertebrate Viruses, Primate Lentiviruses, North and Central America, Viral Sexually Transmitted Diseases, Immune System Diseases and Conditions
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