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CDC HIV/AIDS/Viral Hepatitis/STD/TB Prevention News Update
Inhibition of HIV Replication by Dominant Negative Mutants of
Reddy, Thipparthi R.; Xu, Weidong; Mau, Jonathan K.L.; et
June 21, 1999
Nature Medicine (06/99) Vol. 5, No. 6, P. 635

A study of Sam68 (Src-associated protein in mitosis) and a mutant Rev protein, RevM10, indicate potential methods for viral gene therapy. Investigators found that Sam68 specifically interacts with the Rev response element (RRE) and can partially replace and synergize with Rev in RRE-mediated gene expression and virus replication. According to the data, c-terminally deleted mutants of Sam68 blocked the transactivation of RRE-mediated expression by wild-type Sam68 and Rev. The mutants, which hampered the nuclear localization of Rev in co-expressed cells, stopped wild-type HIV-1 replication as well as the RevM10 mutant. The researchers suggest they may be useful in anti-AIDS treatments.

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