CDC HIV/AIDS/Viral Hepatitis/STD/TB Prevention News Update Measurement of Sputum Mycobacterium Tuberculosis Messenger RNA
Desjardin, Lucy E.; Perkins, Mark D.; Wolski, Kathy; et al.
July 26, 1999
American Journal of Respiratory and Critical Care Medicine
In an effort to identify surrogate markers for response to
chemotherapy, researchers from the United States and Brazil
measured levels of Mycobacterium tuberculosis 85B messenger
RNA (mRNA), 16S ribosomal RNA, and IS6110DNA found in
patients' sputa. According to the authors, the quick
disappearance of M. tuberculosis mRNA from sputum indicates
that it is a useful marker for rapid assessment of response to
chemotherapy and a good indicator of microbial viability.