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Elevated levels of beta-chemokines in bronchoalveolar lavage fluid (BALF) of individuals infected with human T lymphotropic virus type-1 (HTLV-1).
Seki M; Kadota JI; Higashiyama Y; Iida K; Iwashita T; Sasaki E; Maesaki
March 30, 2000
Clin Exp Immunol. 1999 Dec;118(3):417-22. Unique Identifier : AIDSLINE

Pulmonary complications are known to develop in HTLV-1 carriers, including T lymphocytic alveolitis, and increased IL-2 receptor alpha (CD25)-bearing T cells have been found in BALF. Several chemokines may contribute to accumulation of T lymphocytes in the lungs of HTLV-1 carriers. Here, we compared the distribution of T lymphocyte subsets and beta-chemokines, such as macrophage inflammatory peptide-1alpha (MIP-1alpha), regulated on activation normal T expressed and secreted (RANTES), and macrophage chemoattractant protein-1 (MCP-1), in BALF and peripheral blood between HTLV-1 carriers and non-infected healthy normal subjects. Flow cytometric analysis with MoAbs to cell surface antigens was used to identify T lymphocyte subsets in BALF samples from HTLV-1 carriers (n = 13) and non-infected healthy controls (n = 10). The levels of different beta-chemokines were estimated by ELISA. High percentages of CD3+ cells, CD3 expressing HLA-DR antigen and CD3+CD25+ cells were detected in BALF of HTLV-1 carriers compared with non-infected controls. The concentration of MIP-1alpha in BALF of patients was significantly higher than in non-infected healthy controls and correlated well with the percentage of CD3+CD25+ cells. The level of RANTES in BALF was also significantly high in HTLV-1 carriers, but did not correlate with the percentage of CD3+CD25+ cells. On the other hand, the level of MCP-1 in BALF of HTLV-1 carriers was not different from that of controls. Our results suggest a possible interaction between activated T cells bearing CD25 and beta-chemokines, especially MIP-1alpha, which may contribute to the pulmonary involvement in HTLV-1 carriers.

JOURNAL ARTICLE Adult Aged Antigens, CD3/METABOLISM Bronchoalveolar Lavage Fluid/*CHEMISTRY/CYTOLOGY Cell Count Chemokines, CC/*ANALYSIS Enzyme-Linked Immunosorbent Assay Female Flow Cytometry Human HLA-DR Antigens/METABOLISM HTLV-I Infections/*IMMUNOLOGY Lung Diseases/*IMMUNOLOGY Lymphocytosis/IMMUNOLOGY/METABOLISM/PATHOLOGY Macrophage Inflammatory Protein-1/ANALYSIS Male Middle Age Monocyte Chemoattractant Protein-1/ANALYSIS Receptors, Interleukin-2/METABOLISM RANTES/ANALYSIS T-Lymphocyte Subsets/CYTOLOGY/IMMUNOLOGY/METABOLISM