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IS1245 genotypic analysis of Mycobacterium avium isolates from patients in Brazil.
Saad MH; Fonseca LD; Ferrazoli L; Fandinho F; Palaci M; Grinsztejn B;
March 30, 2000
Int J Infect Dis. 1999 Summer;3(4):192-6. Unique Identifier : AIDSLINE

OBJECTIVE: Disseminated Mycobacterium avium infection is an emerging opportunistic disease among patients with acquired immunodeficiency syndrome (AIDS) in Brazil. The mode of transmission of M. avium in a developing country setting needs to be better characterized. METHODS: Mycobacterium avium strain collections in Sao Paulo and Rio de Janeiro were analyzed according to the strains' IS1245 DNA gel electrophoretic migration patterns. Medical records of the patients from whom M. avium isolates were available were reviewed, and their demographic characteristics were stratified according to the isolates' IS1245 DNA fingerprint patterns. RESULTS: Of 105 patients, 33 (31%) with M. avium isolated between 1990 and 1994 had strains having IS1245 patterns identical in patterns seen in isolates from two or more patients (designated as cluster pattern strains). Cluster pattern strains were isolated from 21 (39%) of 54 patients with disseminated infection (defined as infection due to M. avium isolated from a sterile site in an adult patient). Six of the cluster pattern strains were isolated only from sterile sites. In Sao Paulo, cluster pattern strains were significantly more likely to be isolated from patients with disseminated disease. CONCLUSIONS: These preliminary observations suggest that in large cities of Brazil, a high proportion (at least 39%) of disseminated M. avium infections in patients with AIDS results from a recent transmission. Some strains of M. avium may be more likely to cause disseminated disease than others after an infection.

JOURNAL ARTICLE Adolescence Adult AIDS-Related Opportunistic Infections/*MICROBIOLOGY/TRANSMISSION Bacterial Typing Techniques Blood/MICROBIOLOGY Brazil/EPIDEMIOLOGY Child Child, Preschool DNA Fingerprinting *DNA Transposable Elements Female Genotype Human Male Middle Age Mycobacterium avium Complex/CLASSIFICATION/*GENETICS/ISOLATION & PURIF Mycobacterium avium-intracellulare Infection/*MICROBIOLOGY/ TRANSMISSION Retrospective Studies Sputum/MICROBIOLOGY Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S.