Carbohydr Res. 1999 Sep 15;321(1-2):121-7. Unique Identifier : AIDSLINE
A new polysaccharide composed of galactan sulfate with a
beta-(1-->3)-glycosidic linkage has been isolated from the marine clam
species Meretrix petechialis. The polysaccharide was homogeneous in its
composition containing D-galactose. The glycosidic linkage was examined
by 2D DQF-COSY and 2D NOESY spectroscopy. The coupling constant of
anomeric proton was 7.8 Hz, suggesting a beta-galacto configuration. The
downfield shift of H-2 of galactose residue demonstrated the presence of
2-O-sulfonate group. TQF-COSY confirmed that the C-6 position was
substituted with a sulfonate group. The anti-HIV activity of the
polysaccharides has been evaluated by the inhibition of syncytia
formation. The fusion index and percentage fusion inhibition of sulfated
galactan were 0.34 and 56% at 200 micrograms/mL.
JOURNAL ARTICLE Animal Anti-HIV Agents/*ISOLATION & PURIF
Carbohydrate Sequence Clams/*CHEMISTRY Dextran Sulfate/PHARMACOLOGY
Galactans/*ISOLATION & PURIF/PHARMACOLOGY Giant Cells/DRUG EFFECTS
Hela Cells Human HIV-1/DRUG EFFECTS Membrane Fusion/DRUG EFFECTS
Molecular Sequence Data Nuclear Magnetic Resonance Support, Non-U.S.
Gov't Support, U.S. Gov't, P.H.S.