translation agency

Linkage of foreign carrier protein to a self-tumor antigen enhances the immunogenicity of a pulsed dendritic cell vaccine.
Timmerman JM; Levy R; Division of Oncology, Department of Medicine,
July 30, 2000
DOSAGE/IMMUNOLOGY/METABOLISM J Immunol. 2000 May 1;164(9):4797-803.

Mannose-binding lectin (MBL) is present in human serum and plays an important role in innate immunity by binding to carbohydrate on micro-organisms. Whereas the gp120/gp41 of human immunodeficiency virus type 1 (HIV-1) contains numerous N-linked glycosylation sites and many of these sites contain high-mannose glycans which could interact with MBL, the interaction between MBL and primary isolates (PI) of HIV-1 has not been studied. To determine if PI of HIV bind to MBL, a virus capture assay was developed in which virus was incubated in MBL-coated microtitre wells followed by detection of bound virus with an ELISA for p24 antigen. The X4 HIV-1(MN) T cell line-adapted strain and PI of HIV (R5 and X4) bound to MBL. Binding of virus to MBL was via the carbohydrate-recognition domain of MBL since binding did not occur in the absence of Ca(2+) and was blocked by preincubation of MBL-coated wells with soluble mannan. The interaction of virus with MBL-coated wells was also inhibited by preincubation of virus with soluble MBL, indicating that both immobilized and soluble forms of MBL bound to HIV. Although host cell glycoproteins are incorporated into the membrane of HIV, binding of virus to immobilized MBL required expression of gp120/gp41 on virus particles, suggesting the presence of either an unusually high carbohydrate density and/or a unique carbohydrate structure on gp120/gp41 that is the target of MBL. This study shows that PI of HIV bind to MBL and suggests that MBL can selectively interact with HIV in vivo via carbohydrate structures on gp120/gp41.

JOURNAL ARTICLE Adjuvants, Immunologic/*ADMINISTRATION & DOSAGE/METABOLISM Adoptive Transfer Animal Antigens, Neoplasm/ADMINISTRATION & DOSAGE/*IMMUNOLOGY/ *METABOLISM Cancer Vaccines/ADMINISTRATION & DOSAGE/*IMMUNOLOGY/METABOLISM Carrier Proteins/ADMINISTRATION & DOSAGE/*IMMUNOLOGY/*METABOLISM Chimeric Proteins/ADMINISTRATION & DOSAGE/IMMUNOLOGY CD4-Positive T-Lymphocytes/IMMUNOLOGY CD8-Positive T-Lymphocytes/IMMUNOLOGY Dendritic Cells/*IMMUNOLOGY/TRANSPLANTATION Female Granulocyte Macrophage Colony-Stimulating Factors, Recombinant/ ADMINISTRATION & DOSAGE/IMMUNOLOGY/METABOLISM Hemocyanin/ADMINISTRATION & DOSAGE/IMMUNOLOGY/METABOLISM Human Immunoglobulin Idiotypes/ADMINISTRATION & DOSAGE/GENETICS/ METABOLISM Immunoglobulin Isotypes/ADMINISTRATION & DOSAGE/BIOSYNTHESIS Lymphoma/IMMUNOLOGY/PREVENTION & CONTROL Mice Mice, Inbred C3H Neoplasm Transplantation Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Th1 Cells/IMMUNOLOGY Tumor Cells, Cultured Vaccines, Conjugate/ADMINISTRATION & DOSAGE/IMMUNOLOGY/METABOLISM J Immunol. 2000 May 1;164(9):4797-803. Unique Identifier : AIDSLINE MED/20243583