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Cutting edge: CD4 is not required for the functional activity of IL-16.
Mathy NL; Bannert N; Norley SG; Kurth R; Paul-Ehrlich Institute, Langen,
July 30, 2000
J Immunol. 2000 May 1;164(9):4429-32. Unique Identifier : AIDSLINE

IL-16 functions as a chemoattractant factor, inhibitor of HIV replication, and inducer of proinflammatory cytokine production. Previous studies have suggested that CD4 is the receptor for IL-16, because only CD4+ cells respond to IL-16 and both the anti-CD4 Ab OKT4 and soluble CD4 can block IL-16 function. However, these are only indirect evidence of a requirement for CD4, and to date a direct interaction between IL-16 and CD4 has not been shown. In this paper, we report that cells from CD4 knockout mice are as responsive to IL-16 as their CD4 wild-type equivalents in both assays testing for IL-16 function (chemotaxis and production of proinflammatory cytokines). In addition, the inhibitory effect of soluble CD4 on IL-16 function observed using CD4 wild type murine cells was not observed using CD4 knockout cells. These data demonstrate that CD4 is not required for IL-16 function and suggest that another molecule acts as the major receptor.

JOURNAL ARTICLE Animal Antigens, CD4/GENETICS/*PHYSIOLOGY Cells, Cultured Chemotaxis, Leukocyte/IMMUNOLOGY Cytokines/*SECRETION Interleukin-1/SECRETION Interleukin-16/*PHYSIOLOGY Interleukin-6/SECRETION Leukocytes, Mononuclear/IMMUNOLOGY Mice Mice, Inbred C57BL Mice, Knockout Monocytes/IMMUNOLOGY/SECRETION Support, Non-U.S. Gov't Tumor Necrosis Factor/SECRETION