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Regulation of human interleukin-2 gene: functional DNA sequences in the 5' flanking region for the gene expression in activated T lymphocytes.
Fujita T; Shibuya H; Ohashi T; Yamanishi K; Taniguchi T
November 30, 1986
Cell. 1986 Aug 1;46(3):401-5. Unique Identifier : AIDSLINE MED/86272087

Interleukin-2 (IL-2) is a lymphokine that plays a crucial role in the immune system, especially in the growth control of T lymphocytes. Expression of this lymphokine is restricted to activated T lymphocytes. Here we demonstrate the presence of unique DNA sequences in the 5' flanking region of the human IL-2 gene that control induced T-cell-specific gene expression. We also show that the DNA sequences function in an orientation-independent manner and activate a heterologous promoter which is otherwise inert in induced T cells. The DNA, which spans about 200 bp, contains regions with sequence homology to LTR sequences of HTLV-III (or LAV) and the 5' upstream region of the IL-2 receptor and interferon-gamma genes.

Base Sequence Cell Line DNA/*GENETICS Enhancer Elements (Genetics) *Gene Expression Regulation Genes, Synthetic Human HTLV-BLV Viruses/GENETICS Interleukin-2/BIOSYNTHESIS/*GENETICS Lymphocyte Transformation Promoter Regions (Genetics) Sequence Homology, Nucleic Acid Support, Non-U.S. Gov't T-Lymphocytes/METABOLISM JOURNAL ARTICLE