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Model system in HIV-antibody testing for Boehringer Mannheim Enzymun-Test diagnostics.
Bayer H; Lin M; Klein C; Kirch P; Boehringer Mannheim GmbH,
September 30, 1990
Int Conf AIDS. 1989 Jun 4-9;5:487 (abstract no. B.542). Unique

Screening tests have to be sensitive for possibly all specificities of HIV-antibodies, while specific antibody tests are important for prognosis of diseases (p24) or distinguishing HIV-I from HIV-II-infection. For our automatic Boehringer Mannheim Enzymun-Test-System we developed a model-system using a single universal biotin binding solid phase in all tests and combination of specific biotin labelled antigens for the individual tests. Polystyrene tubes were coated with streptavidin. Recombinant antigens were labelled with biotin. Synthetic peptides were synthesized by solid phase technology. The samples were incubated with the antigens in PBS containing bovine serum. Solid phase bound complexes were detected by peroxidase labelled second antibodies and visualized using ABTS substrate. We showed that the model system is suited for detection of p24 specific antibodies in a quantitative test with the linear standard curve in high precision (less than 3 %) as well as for screening tests to HIV-I and HIV-II using recombinant HIV-I gp41 and p24 antigens and synthetic peptides from HIV-II gp32.

Biotin Gene Products, gag/IMMUNOLOGY Human HIV Antibodies/*ANALYSIS HIV Envelope Protein gp41/IMMUNOLOGY HIV Infections/*DIAGNOSIS HIV-1/*IMMUNOLOGY HIV-2/*IMMUNOLOGY *Immunoassay Polystyrenes Prognosis Recombinant Proteins/IMMUNOLOGY Sensitivity and Specificity Viral Core Proteins/IMMUNOLOGY ABSTRACT