Int Conf AIDS. 1990 Jun 20-23;6(3):115 (abstract no. S.A.78). Unique
OBJECTIVE: To study protective immunity to HIV-2 in an animal model.
METHODS: 4 cynomolgus monkeys were immunized with killed HIV-2
(SBL-6669), 2 of them with 5 injections of 100 or 300 mug viral protein
in Freunds incomplete adjuvans (FA) and the other 2 animals with 4
injections of 25 or 50 mug viral protein in iscoms. Challenge with 100
animal infectious doses of live HIV-2(SBL-6669) was done 2 weeks after
the last booster. 4 control animals received the same dose of live
virus. Virus isolations, determination of viral antigen by ELISA and of
antibodies by ELISA, Western blot and radioimmunoprecipitation were
done. RESULTS: All 4 immunized monkeys developed good antibody responses
to HIV-2 envelope and core antigens. The 2 monkeys immunized with virus
in FA showed no evidence of infection after challenge with live virus
(no virus recovery and no viral antigen). In contrast virus was
recovered repeatedly during the first month after challenge in all 4
non-immunized animals and once in the 2 animals immunized with iscoms.
Viral antigen was demonstrated in blood of 3 of 4 challenged control
animals. CONCLUSION: The demonstration of vaccine induced protection
against HIV-2 infection in monkeys provide hope for vaccination against
HIV also in man.
Acquired Immunodeficiency Syndrome/*IMMUNOLOGY Animal HIV
Antibodies/IMMUNOLOGY HIV Antigens/IMMUNOLOGY HIV-2/*IMMUNOLOGY
Macaca fascicularis Vaccination Vaccines, Inactivated/*IMMUNOLOGY
Viral Proteins/IMMUNOLOGY Viral Vaccines/*IMMUNOLOGY ABSTRACT