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AIDS Kaposi's sarcoma-derived cells produce and respond to interleukin 6.
Miles SA; Rezai AR; Logan D; Salazar-Gonzalez JF; Vander Meyden M;
December 30, 1990
Int Conf AIDS. 1990 Jun 20-23;6(3):112 (abstract no. S.A.66). Unique

OBJECTIVE: To determine the relationship of interleukin 6 to the development of AIDS related Kaposi's sarcoma. METHODS: Cell lines derived from Kaposi's Sarcoma lesions of patients with AIDS (AIDS-KS cells) were examined for the production of IL-6 using IL-6 bioassay, immunoassay and immunoperoxidase staining. IL-6 and IL-6 receptor expression was examined by RNA slot blot hybridization. Fresh autopsy material from patients with AIDS/KS was examined for RNA production. RESULTS: The AIDS-KS cell lines (N521J and EKS 3) secreted large amounts of immunoreactive and biologically active IL-6. We found both IL-6 and IL-6 receptor (IL-6-R) RNA by slot blot hybridization analysis of these cells. The IL-6 receptor was functional, as 3H-thymidine incorporation by N521J cells increased significantly after exposure to greater than 10 units/ml of recombinant human IL-6 (rhIL-6). Antisense IL-6 oligonucleotides inhibit cell proliferation which was reversed by exogenous IL-6. We confirmed that AIDS-KS cells produced IL-6 in vivo by preparing RNA and tissue sections from involved (KS) and uninvolved skin from a patient with AIDS-KS. We detected immunoreactive IL-6 in the involved tumor areas and to a lesser extent in the surrounding normal epidermis. Slot blot hybridization showed a great excess of IL-6 and IL-6-R RNA in involved skin compared to uninvolved skin. CONCLUSIONS: These results show that both IL-6 and IL-6-R are produced by AIDS-KS cells and suggest that IL-6 may be a growth factor for AIDS-KS cells in vitro.

Acquired Immunodeficiency Syndrome/COMPLICATIONS/*GENETICS/ PATHOLOGY Blotting, Northern Human Interleukin-6/BIOSYNTHESIS/*GENETICS Receptors, Immunologic/DRUG EFFECTS/GENETICS RNA, Messenger/ANTAGONISTS & INHIB Sarcoma, Kaposi's/ETIOLOGY/*GENETICS Tumor Cells, Cultured ABSTRACT