AIDS Res Hum Retroviruses. 1990 Dec;6(12):1433-6. Unique Identifier :
Infectivity of free and cell-associated human immunodeficiency virus
type 1 (HIV-1) treated in vitro at pH 7.4 to 4.9 for 2 hours was
assessed on susceptible CEM-ss cells. Viral activity was monitored by
cytopathology and production of reverse transcriptase and p24 antigen.
The infectivity of cell-free virus was gradually inactivated and at pH
5.4 was completely lost, with or without subsequent adjustment of pH to
neutral. Virus-producing cells also gradually lost their ability to
infect as the pH decreased; however, restoration of neutral pH resulted
in regained infectivity. Since the pH values used in the study are
similar to those found at various entry sites of the human body, the
data may be relevant to the mode of transmittal of HIV.
Cell Line Cell Survival Cell-Free System Human Hydrogen-Ion
Concentration HIV-1/*PHYSIOLOGY Support, Non-U.S. Gov't *Virus
Replication JOURNAL ARTICLE