J Virol. 1991 Jun;65(6):2895-902. Unique Identifier : AIDSLINE
Human herpesvirus 6 (HHV-6) can activate the human immunodeficiency
virus (HIV) promoter and accelerate cytopathic effects in HIV-infected
human T cells. This study examines the regions of the HIV promoter
required for HHV-6 transactivation in a heterogeneous population of
primary human T lymphocytes with or without antigenic stimulation. Two
different strains of HHV-6, GS and Z29, transactivated the HIV promoter.
The GS strain transactivated the promoter in both stimulated and resting
T cells, while the Z29 strain increased HIV promoter activity only in
stimulated T cells. Three DNA clones containing HHV-6(GS) genomic
fragments transactivated the HIV promoter in cotransfected T cells. A
21.4-kb DNA clone, pZVB70, showed the highest transactivating ability,
while two other DNA fragments, pZVB10 (6.2 kb) and pZVH14 (8.7 kb),
showed lower activity. One of these clones, pZVH14, activated the HIV
promoter construct containing a mutation in the NF kappa B site.
However, this mutated NF kappa B promoter was not transactivated during
HHV-6(GS) infection or after cotransfection with pZVB70 or pZVB10. These
data indicate that the NF kappa B sites of the HIV promoter are
essential for its transactivation during HHV-6(GS) infection. By
increasing HIV promoter activity in primary T lymphocytes, HHV-6 may
consequently increase HIV replication, leading to an increase in the
cytopathic effect on coinfected human T cells.
Base Sequence Cells, Cultured Chloramphenicol
Acetyltransferase/METABOLISM Cytopathogenic Effect, Viral DNA,
Viral/CHEMISTRY Enhancer Elements (Genetics) *Gene Expression
Regulation, Viral Herpesviridae Infections/GENETICS Herpesvirus 6,
Human/*GENETICS Human HIV/GROWTH & DEVELOPMENT/*GENETICS HIV Long
Terminal Repeat Molecular Sequence Data NF-kappa B/GENETICS *Promoter
Regions (Genetics) Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S.
T-Lymphocytes/*MICROBIOLOGY Transfection Virus Replication JOURNAL